Single-cell microfluidic impedance cytometry: from raw signals to cell phenotypes using data analytics.

ABSTRACT: The biophysical analysis of single-cells by microfluidic impedance cytometry is emerging as a label-free and high-throughput means to stratify the heterogeneity of cellular systems based on their electrophysiology. Emerging applications range from fundamental life-science and drug assessment research to point-of-care diagnostics and precision medicine. Recently, novel chip designs and data analytic strategies are laying the foundation for multiparametric cell characterization and subpopulation distinction, which are essential to understand biological function, follow disease progression and monitor cell behaviour in microsystems. In this tutorial review, we present a comparative survey of the approaches to elucidate cellular and subcellular features from impedance cytometry data, covering

SUBMITTER: Honrado C 

PROVIDER: S-EPMC7909465 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature