Bacillus thuringiensis Cells Selectively Captured by Phages and Identified by Surface Enhanced Raman Spectroscopy Technique.

ABSTRACT: In this work, the results on the detection and identification of Bacillus thuringiensis (Bt) cells by using surface-enhanced Raman spectroscopy (SERS) are presented. Bt has been chosen as a harmless surrogate of the pathogen Bacillus anthracis (Ba) responsible for the deadly Anthrax disease, because of their genetic similarities. Drops of 200 μL of Bt suspensions, with concentrations 102 CFU/mL, 104 CFU/mL, 106 CFU/mL, were deposited on a SERS chip and sampled after water evaporation. To minimize the contribution to the SERS data given by naturally occurring interferents present in a real scenario, the SERS chip was functionalized with specific phage receptors BtCS33, that bind Bt (or Ba) cells to the SERS surface and allow to rinse the chip removing unwanted contaminants. Different chemom

SUBMITTER: Almaviva S 

PROVIDER: S-EPMC7909556 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature