Relative qPCR to quantify colonization of plant roots by arbuscular mycorrhizal fungi.

ABSTRACT: Arbuscular mycorrhiza fungi (AMF) are beneficial soil fungi that can promote the growth of their host plants. Accurate quantification of AMF in plant roots is important because the level of colonization is often indicative of the activity of these fungi. Root colonization is traditionally measured with microscopy methods which visualize fungal structures inside roots. Microscopy methods are labor-intensive, and results depend on the observer. In this study, we present a relative qPCR method to quantify AMF in which we normalized the AMF qPCR signal relative to a plant gene. First, we validated the primer pair AMG1F and AM1 in silico, and we show that these primers cover most AMF species present in plant roots without amplifying host DNA. Next, we compared the relative qPCR method with trad

SUBMITTER: Bodenhausen N 

PROVIDER: S-EPMC7910240 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature