Probing hepatic metabolism of [2-¹³C]dihydroxyacetone in vivo with ¹H-decoupled hyperpolarized ¹³C-MR.

ABSTRACT:

Objectives

To enhance detection of the products of hyperpolarized [2-¹³C]dihydroxyacetone metabolism for assessment of three metabolic pathways in the liver in vivo. Hyperpolarized [2-¹³C]DHAc emerged as a promising substrate to follow gluconeogenesis, glycolysis and the glycerol pathways. However, the use of [2-¹³C]DHAc in vivo has not taken off because (i) the chemical shift range of [2-¹³C]DHAc and its metabolic products span over 144 ppm, and (ii) ¹H decoupling is required to increase spectral resolution and sensitivity. While these issues are trivial for high-field vertical-bore NMR spectrometers, horizontal-bore small-animal MR scanners are seldom equipped for such experiments.

Methods

Real-time hepatic metabolism

SUBMITTER: Marco-Rius I 

PROVIDER: S-EPMC7910257 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature