An alternative food source for metabolism and longevity studies in Caenorhabditis elegans.

ABSTRACT: Caenorhabditis elegans is an instrumental research model used to advance our knowledge in areas including development, metabolism, and aging. However, research on metabolism and/or other measures of health/aging are confounded by the nematode's food source in the lab, live E. coli bacteria. Commonly used treatments, including ultraviolet irradiation and antibiotics, are successful in preventing bacterial replication, but the bacteria can remain metabolically active. The purpose of this study is to develop a metabolically inactive food source for the worms that will allow us to minimize the confounding effects of bacterial metabolism on worm metabolism and aging. Our strategy is to use a paraformaldehyde (PFA) treated E. coli food source and to determine its effects on worm health, metaboli

SUBMITTER: Beydoun S 

PROVIDER: S-EPMC7910432 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature