Morphometric Analysis of Retinal Pigment Epithelial Cells From C57BL/6J Mice During Aging.

ABSTRACT:

Purpose

To quantitatively evaluate the changes in orientation and morphometric features of mouse retinal pigment epithelial (RPE) cells in different regions of the eye during aging.

Methods

We segmented individual RPE cells from whole RPE flatmount images of C57BL/6J mice (postnatal days 30 to 720) using a machine-learning method and evaluated changes in morphometric features, including our newly developed metric combining alignment and shape of RPE cells during aging.

Results

Mainly, the anterior part of the RPE sheet grows during aging, while the posterior part remains constant. Changes in size and shape of the peripheral RPE cells are prominent with aging as cells become larger, elongated, and concave. Conversely, the central RPE cells maintain relatively constant

SUBMITTER: Kim YK 

PROVIDER: S-EPMC7910641 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature