Repopulation of decellularised articular cartilage by laser-based matrix engraving.

ABSTRACT:

Background

In spite of advances in the treatment of cartilage defects using cell and scaffold-based therapeutic strategies, the long-term outcome is still not satisfying since clinical scores decline years after treatment. Scaffold materials currently used in clinical settings have shown limitations in providing suitable biomechanical properties and an authentic and protective environment for regenerative cells. To tackle this problem, we developed a scaffold material based on decellularised human articular cartilage.

Methods

Human articular cartilage matrix was engraved using a CO₂ laser and treated for decellularisation and glycosaminoglycan removal. Characterisation of the resulting scaffold was performed via mechanical testing, DNA and GAG quantification and i

SUBMITTER: Nurnberger S 

PROVIDER: S-EPMC7910698 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature