Accelerating 3D single-molecule localization microscopy using blind sparse inpainting.

ABSTRACT: Single-molecule localization-based super-resolution microscopy has enabled the imaging of microscopic objects beyond the diffraction limit. However, this technique is limited by the requirements of imaging an extremely large number of frames of biological samples to generate a super-resolution image, thus requiring a longer acquisition time. Additionally, the processing of such a large image sequence leads to longer data processing time. Therefore, accelerating image acquisition and processing in single-molecule localization microscopy (SMLM) has been of perennial interest. To accelerate three-dimensional (3D) SMLM imaging by leveraging a computational approach without compromising the resolution. We used blind sparse inpainting to reconstruct high-density 3D images from low-density ones.

SUBMITTER: Gaire SK 

PROVIDER: S-EPMC7910702 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature