Method for the Detection of the Cleaved Form of Shiga Toxin 2a Added to Normal Human Serum.

ABSTRACT: The pathogenesis of Escherichia coli-induced hemolytic uremic syndrome (eHUS) caused by infections with pathogenic Shiga toxin (Stx) producing E. coli (STEC) is centered on bacterial (e.g., Stx) and host factors (circulating cells, complement system, serum proteins) whose interaction is crucial for the immediate outcome and for the development of this life-threatening sequela. Stx2a, associated to circulating cells (early toxemia) or extracellular vesicles (late toxemia) in blood, is considered the main pathogenic factor in the development of eHUS. Recently, it was found that the functional properties of Stx2a (binding to circulating cells and complement components) change according to modifications of the structure of the toxin, i.e., after a single cleavage of the A subunit resulting in

SUBMITTER: Rocchetti L 

PROVIDER: S-EPMC7911550 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature