Five Silkworm 30K Proteins Are Involved in the Cellular Immunity against Fungi.

ABSTRACT:

Background

30K proteins are a major group of nutrient storage proteins in the silkworm hemolymph. Previous studies have shown that 30K proteins are involved in the anti-fungal immunity; however, the molecular mechanism involved in this immunity remains unclear.

Methods

We investigated the transcriptional expression of five 30K proteins, including BmLP1, BmLP2, BmLP3, BmLP4, and BmLP7. The five recombinant 30K proteins were expressed in an Escherichia coli expression system, and used for binding assays with fungal cells and hemocytes.

Results

The transcriptional expression showed that the five 30K proteins were significantly upregulated after injection of pathogen-associated molecular patterns to the fifth instar larvae, indicating the possibility of their invol

SUBMITTER: Ye L 

PROVIDER: S-EPMC7911669 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature