Application of Real-Time PCR for the Detection and Quantification of Oomycetes in Ornamental Nursery Stock.

ABSTRACT: Numerous Phytophthora and Pythium disease outbreaks have occurred in Europe following inadvertent introduction of contaminated ornamental plants. Detection and identification of pathogens are crucial to reduce risks and improve plant biosecurity in Europe and globally. Oomycete diversity present in roots and compost was determined in 99 hardy woody plants bought from nurseries, retailers and internet sellers, using both isolations and molecular analyses. Oomycete DNA was quantified using real-time PCR of environmental DNA from the plants using three loci: ITS, trnM-trnP-trnM and atp9-nad9. At least one oomycete species was isolated from 89.9% of plants using classical techniques. In total, 10 Phytophthora spp., 17 Pythium spp. and 5 Phytopythium spp. were

SUBMITTER: Puertolas A 

PROVIDER: S-EPMC7911672 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature