Modeling of Hypoxic Brain Injury through 3D Human Neural Organoids.

ABSTRACT: Brain organoids have emerged as a novel model system for neural development, neurodegenerative diseases, and human-based drug screening. However, the heterogeneous nature and immature neuronal development of brain organoids generated from pluripotent stem cells pose challenges. Moreover, there are no previous reports of a three-dimensional (3D) hypoxic brain injury model generated from neural stem cells. Here, we generated self-organized 3D human neural organoids from adult dermal fibroblast-derived neural stem cells. Radial glial cells in these human neural organoids exhibited characteristics of the human cerebral cortex trend, including an inner (ventricular zone) and an outer layer (early and late cortical plate zones). These data suggest that neural organoids reflect the distinctive ra

SUBMITTER: Kim MS 

PROVIDER: S-EPMC7911731 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature