The Ustilago hordei-Barley Interaction Is a Versatile System for Characterization of Fungal Effectors.

ABSTRACT: Obligate biotrophic fungal pathogens, such as Blumeria graminis and Puccinia graminis, are amongst the most devastating plant pathogens, causing dramatic yield losses in many economically important crops worldwide. However, a lack of reliable tools for the efficient genetic transformation has hampered studies into the molecular basis of their virulence or pathogenicity. In this study, we present the Ustilago hordei-barley pathosystem as a model to characterize effectors from different plant pathogenic fungi. We generate U. hordei solopathogenic strains, which form infectious filaments without the presence of a compatible mating partner. Solopathogenic strains are suitable for heterologous expression system for fungal virulence factors. A highly efficient Crispr/

SUBMITTER: Okmen B 

PROVIDER: S-EPMC7912019 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature