Alternative mRNA Processing of Innate Response Pathways in Respiratory Syncytial Virus (RSV) Infection.

ABSTRACT: The innate immune response (IIR) involves rapid genomic expression of protective interferons (IFNs) and inflammatory cytokines triggered by intracellular viral replication. Although the transcriptional control of the innate pathway is known in substantial detail, little is understood about the complexity of alternative splicing (AS) and alternative polyadenylation (APA) of mRNAs underlying the cellular IIR. In this study, we applied single-molecule, real-time (SMRT) sequencing with mRNA quantitation using short-read mRNA sequencing to characterize changes in mRNA processing in the epithelial response to respiratory syncytial virus (RSV) replication. Mock or RSV-infected human small-airway epithelial cells (hSAECs) were profiled using SMRT sequencing and the curated transcriptome analyzed b

SUBMITTER: Xu X 

PROVIDER: S-EPMC7912025 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature