Multiscale Electron Microscopy for the Study of Viral Replication Organelles.

ABSTRACT: During infection with positive-strand RNA viruses, viral RNA synthesis associates with modified intracellular membranes that form unique and captivating structures in the cytoplasm of the infected cell. These viral replication organelles (ROs) play a key role in the replicative cycle of important human pathogens like coronaviruses, enteroviruses, or flaviviruses. From their discovery to date, progress in our understanding of viral ROs has closely followed new developments in electron microscopy (EM). This review gives a chronological account of this progress and an introduction to the different EM techniques that enabled it. With an ample repertoire of imaging modalities, EM is nowadays a versatile technique that provides structural and functional information at a wide range of scales. Tog

SUBMITTER: Wolff G 

PROVIDER: S-EPMC7912242 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature