Osthole enhances the bone mass of senile osteoporosis and stimulates the expression of osteoprotegerin by activating β-catenin signaling.

ABSTRACT:

Introduction

Osthole has a potential therapeutic application for anti-osteoporosis. The present study verified whether osthole downregulates osteoclastogenesis via targeting OPG.

Methods

In vivo, 12-month-old male mice were utilized to evaluate the effect of osthole on bone mass. In vitro, bone marrow stem cells (BMSCs) were isolated and extracted from 3-month-old OPG^-/- mice and the littermates of OPG^+/+ mice. Calvaria osteoblasts were extracted from 3-day-old C57BL/6J mice or 3-day-old OPG^-/- mice and the littermates of OPG^+/+ mice.

Results

Osthole significantly increased the gene and protein levels of OPG in primary BMSCs in a dose-dependent manner. The deletion of the OPG gene did not affect β-catenin expression. The del

SUBMITTER: Jin ZX 

PROVIDER: S-EPMC7912492 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature