A Perfusion Bioreactor for Longitudinal Monitoring of Bioengineered Liver Constructs.

ABSTRACT: In the field of in vitro liver disease models, decellularised organ scaffolds maintain the original biomechanical and biological properties of the extracellular matrix and are established supports for in vitro cell culture. However, tissue engineering approaches based on whole organ decellularized scaffolds are hampered by the scarcity of appropriate bioreactors that provide controlled 3D culture conditions. Novel specific bioreactors are needed to support long-term culture of bioengineered constructs allowing non-invasive longitudinal monitoring. Here, we designed and validated a specific bioreactor for long-term 3D culture of whole liver constructs. Whole liver scaffolds were generated by perfusion decellularisation of rat livers. Scaffolds were seeded with Luc+HepG2 and primary human he

SUBMITTER: Sassi L 

PROVIDER: S-EPMC7912543 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature