Quiescence, Stemness and Adipogenic Differentiation Capacity in Human DLK1^-/CD34⁺/CD24⁺ Adipose Stem/Progenitor Cells.

ABSTRACT: We explore the status of quiescence, stemness and adipogenic differentiation capacity in adipose stem/progenitor cells (ASCs) ex vivo, immediately after isolation from human subcutaneous white adipose tissue, by sorting the stromal vascular fraction into cell-surface DLK1⁺/CD34^-, DLK1⁺/CD34^dim and DLK1^-/CD34⁺ cells. We demonstrate that DLK1^-/CD34⁺ cells, the only population exhibiting proliferative and adipogenic capacity, express ex vivo the bonafide quiescence markers p21^Cip1, p27^Kip1 and p57^Kip2 but neither proliferation markers nor the senescence marker p16^Ink4a. The pluripotency markers NANOG, SOX2 and OCT4 are barely detectable in ex vivo ASCs while the

SUBMITTER: Hatzmann FM 

PROVIDER: S-EPMC7912596 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature