Detection of gene fusions using targeted next-generation sequencing: a comparative evaluation.

ABSTRACT:

Background

Gene fusions represent promising targets for cancer therapy in lung cancer. Reliable detection of multiple gene fusions is therefore essential.

Methods

Five commercially available parallel sequencing assays were evaluated for their ability to detect gene fusions in eight cell lines and 18 FFPE tissue samples carrying a variety of known gene fusions. Four RNA-based assays and one DNA-based assay were compared; two were hybrid capture-based, TruSight Tumor 170 Assay (Illumina) and SureSelect XT HS Custom Panel (Agilent), and three were amplicon-based, Archer FusionPlex Lung Panel (ArcherDX), QIAseq RNAscan Custom Panel (Qiagen) and Oncomine Focus Assay (Thermo Fisher Scientific).

Results

The Illumina assay detected all tested fusions and showed the smallest n

SUBMITTER: Heydt C 

PROVIDER: S-EPMC7912891 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature