Elucidating the cellular mechanism for E2-induced dermal fibrosis.

ABSTRACT:

Background

Both TGFβ and estradiol (E2), a form of estrogen, are pro-fibrotic in the skin. In the connective tissue disease, systemic sclerosis (SSc), both TGFβ and E2 are likely pathogenic. Yet the regulation of TGFβ in E2-induced dermal fibrosis remains ill-defined. Elucidating those regulatory mechanisms will improve the understanding of fibrotic disease pathogenesis and set the stage for developing potential therapeutics. Using E2-stimulated primary human dermal fibroblasts in vitro and human skin tissue ex vivo, we identified the important regulatory proteins for TGFβ and investigated the extracellular matrix (ECM) components that are directly stimulated by E2-induced TGFβ signaling.

Methods

We used primary human dermal fibroblasts in vitro and human skin tissue ex vivo

SUBMITTER: Baker Frost D 

PROVIDER: S-EPMC7913437 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature