Pilot study on the effects of preservatives on corneal collagen parameters measured by small angle X-ray scattering analysis.

ABSTRACT:

Objective

Small angle X-ray scattering (SAXS) analysis is a sensitive way of determining the ultrastructure of collagen in tissues. Little is known about how parameters measured by SAXS are affected by preservatives commonly used to prevent autolysis. We determined the effects of formalin, glutaraldehyde, Triton X and saline on measurements of fibril diameter, fibril diameter distribution, and D-spacing of corneal collagen using SAXS analysis.

Results

Compared to sections of sheep and cats' corneas stored frozen as controls, those preserved in 5% glutaraldehyde and 10% formalin had significantly larger mean collagen fibril diameters, increased fibril diameter distribution and decreased D-spacing. Sections of corneas preserved in Triton X had significantly increased collagen

SUBMITTER: Kelly SJ 

PROVIDER: S-EPMC7913446 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature