Generating long-read sequences using Oxford Nanopore Technology from Diospyros celebica genomic DNA.

ABSTRACT:

Objectives

Development of sequencing technology has opened up vast opportunities for tree genomic research in the tropics. One of the aforesaid technologies named ONT (Oxford Nanopore Technology) has attracted researchers in undertaking testings and experiments due to its affordability and accessibility. To the best of our knowledge, there has been no published reports on the use of ONT for genomic analysis of Indonesian tree species. This progress is promising for further improvement in order to acquire more genomic data for research purposes. Therefore, the present study was carried out to determine the effectiveness of ONT in generating long-read DNA sequences using DNA isolated from leaves and wood cores of Macassar ebony (Diospyros celebica Bakh.).

Data description

Long

SUBMITTER: Siregar IZ 

PROVIDER: S-EPMC7913452 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature