Overlapping but distinct: a new model for G-quadruplex biochemical specificity.

ABSTRACT: G-quadruplexes are noncanonical nucleic acid structures formed by stacked guanine tetrads. They are capable of a range of functions and thought to play widespread biological roles. This diversity raises an important question: what determines the biochemical specificity of G-quadruplex structures? The answer is particularly important from the perspective of biological regulation because genomes can contain hundreds of thousands of G-quadruplexes with a range of functions. Here we analyze the specificity of each sequence in a 496-member library of variants of a reference G-quadruplex with respect to five functions. Our analysis shows that the sequence requirements of G-quadruplexes with these functions are different from one another, with some mutations altering biochemical specificity by or

SUBMITTER: Volek M 

PROVIDER: S-EPMC7913677 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature