SUMOylation regulates the protein network and chromatin accessibility at glucocorticoid receptor-binding sites.

ABSTRACT: Glucocorticoid receptor (GR) is an essential transcription factor (TF), controlling metabolism, development and immune responses. SUMOylation regulates chromatin occupancy and target gene expression of GR in a locus-selective manner, but the mechanism of regulation has remained elusive. Here, we identify the protein network around chromatin-bound GR by using selective isolation of chromatin-associated proteins and show that the network is affected by receptor SUMOylation, with several nuclear receptor coregulators and chromatin modifiers preferring interaction with SUMOylation-deficient GR and proteins implicated in transcriptional repression preferring interaction with SUMOylation-competent GR. This difference is reflected in our chromatin binding, chromatin accessibility and gene express

SUBMITTER: Paakinaho V 

PROVIDER: S-EPMC7913686 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature