Novel Pannexin-1-Coupled Signaling Cascade Involved in the Control of Endothelial Cell Function and NO-Dependent Relaxation.

ABSTRACT: Deletion of pannexin-1 (Panx-1) leads not only to a reduction in endothelium-derived hyperpolarization but also to an increase in NO-mediated vasodilation. Therefore, we evaluated the participation of Panx-1-formed channels in the control of membrane potential and [Ca²⁺]_i of endothelial cells. Changes in NO-mediated vasodilation, membrane potential, superoxide anion (O₂ ^·-) formation, and endothelial cell [Ca²⁺]_i were analyzed in rat isolated mesenteric arterial beds and primary cultures of mesenteric endothelial cells. Inhibition of Panx-1 channels with probenecid (1 mM) or the Panx-1 blocking peptide ¹⁰Panx (60 μM) evoked an increase in the ACh (100 nM)-induced vasodilation of KCl-contracted mesenteries a

SUBMITTER: Lillo MA 

PROVIDER: S-EPMC7914086 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature