Serotonin-specific neurons differentiated from human iPSCs form distinct subtypes with synaptic protein assembly.

ABSTRACT: Human induced pluripotent stem cells (hiPSCs) have revolutionized the generation of experimental disease models, but the development of protocols for the differentiation of functionally active neuronal subtypes with defined specification is still in its infancy. While dysfunction of the brain serotonin (5-HT) system has been implicated in the etiology of various neuropsychiatric disorders, investigation of functional human 5-HT specific neurons in vitro has been restricted by technical limitations. We describe an efficient generation of functionally active neurons from hiPSCs displaying 5-HT specification by modification of a previously reported protocol. Furthermore, 5-HT specific neurons were characterized using high-end fluorescence imaging including super-resolution microscopy in combi

SUBMITTER: Jansch C 

PROVIDER: S-EPMC7914246 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature