Central Cholinergic Synapse Formation in Optimized Primary Septal-Hippocampal Co-cultures.

ABSTRACT: Septal innervation of basal forebrain cholinergic neurons to the hippocampus is critical for normal learning and memory and is severely degenerated in Alzheimer's disease. To understand the molecular events underlying physiological cholinergic synaptogenesis and remodeling, as well as pathological loss, we developed an optimized primary septal-hippocampal co-culture system. Hippocampal and septal tissue were harvested from embryonic Sprague-Dawley rat brain and cultured together at varying densities, cell ratios, and in the presence of different growth factors. We identified conditions that produced robust septal-hippocampal synapse formation. We used confocal microscopy with primary antibodies and fluorescent ligands to validate that this system was capable of generating developmentally m

SUBMITTER: Djemil S 

PROVIDER: S-EPMC7914286 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature