Dataset Information

Genomics Analysis of Bacillus megaterium 1259 as a Probiotic and Its Effects on Performance in Lactating Dairy Cows.

ABSTRACT: In this study, we isolated a novel bacterium, Bacillus megaterium 1259 (BM1259), from chicken manure. Whole-genome sequencing analysis showed that the BM1259 complete genome is composed of a 5,043,095 bp circular chromosome and three circular plasmids, and it encodes 5379 coding genes and 182 RNA genes. Among these genes, a series of nitrate assimilation-related genes and pathways were identified, implying a potential role of BM1259 in nitrate metabolism. In addition, 24 lactating Holstein dairy cows were randomly assigned to four groups that were fed a total mixed ration (TMR) diet only (C), a TMR diet supplemented with 5 g/day of BM1259 (T1), a TMR diet supplemented with 10 g/day of BM1259 (T2), or a TMR diet supplemented with 15 g/day of BM1259 (T3). The results showed that supplementing dairy cows with 15 g/day of BM1259 increased 4% fat-corrected milk production. The molar proportion of propionate (C3) was significantly higher in T2 than in C. The C2:C3 ratio of T3 was higher than those of C and T2. No negative effect of BM1259 on blood indicators was detected. This study demonstrates BM1259 can be applied as a potential probiotic to improve nitrogen utilization and milk production in lactating dairy cows.

SUBMITTER: Deng B

PROVIDER: S-EPMC7914491 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature

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Genomics Analysis of <i>Bacillus megaterium</i> 1259 as a Probiotic and Its Effects on Performance in Lactating Dairy Cows.

Deng Bobo B Wang Lin L Ma Qianbo Q Yu Tongshui T Liu Dalin D Dai Yi Y Zhao Guoqi G

Animals : an open access journal from MDPI 20210204 2

In this study, we isolated a novel bacterium, <i>Bacillus megaterium</i> 1259 (BM1259), from chicken manure. Whole-genome sequencing analysis showed that the BM1259 complete genome is composed of a 5,043,095 bp circular chromosome and three circular plasmids, and it encodes 5379 coding genes and 182 RNA genes. Among these genes, a series of nitrate assimilation-related genes and pathways were identified, implying a potential role of BM1259 in nitrate metabolism. In addition, 24 lactating Holstei ...[more]

PMID: 33557352

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Dataset Information

Genomics Analysis of Bacillus megaterium 1259 as a Probiotic and Its Effects on Performance in Lactating Dairy Cows.

Publications

Genomics Analysis of <i>Bacillus megaterium</i> 1259 as a Probiotic and Its Effects on Performance in Lactating Dairy Cows.

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