Generation of the Human Pluripotent Stem-Cell-Derived Astrocyte Model with Forebrain Identity.

ABSTRACT: Astrocytes form functionally and morphologically distinct populations of cells with brain-region-specific properties. Human pluripotent stem cells (hPSCs) offer possibilities to generate astroglia for studies investigating mechanisms governing the emergence of astrocytic diversity. We established a method to generate human astrocytes from hPSCs with forebrain patterning and final specification with ciliary neurotrophic factor (CNTF). Transcriptome profiling and gene enrichment analysis monitored the sequential expression of genes determining astrocyte differentiation and confirmed activation of forebrain differentiation pathways at Day 30 (D30) and D60 of differentiation in vitro. More than 90% of astrocytes aged D95 in vitro co-expressed the astrocytic markers glial fibrillary acidic prot

SUBMITTER: Peteri UK 

PROVIDER: S-EPMC7914711 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature