A Novel Artificially Humanized Anti-Cripto-1 Antibody Suppressing Cancer Cell Growth.

ABSTRACT: Cripto-1 is a member of the EGF-CFC/FRL1/Cryptic family and is involved in embryonic development and carcinogenesis. We designed a novel anti-Cripto-1 artificial antibody and assessed the recognition to the antigen and the potential to suppress the growth of cancer stem cells. First, single chain antibody clones were isolated by bio-panning with the affinity to recombinant Cripto-1 protein from our original phage-display library. Then, the variable regions of heavy chain VH and light chain VL in each clone were fused to constant regions of heavy chain CH and light chain CL regions respectively. These fused genes were expressed in ExpiCHO-S cells to produce artificial humanized antibodies against Cripto-1. After evaluation of the expression levels, one clone was selected and the anti-Cripto

SUBMITTER: Ishii H 

PROVIDER: S-EPMC7915030 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature