Deletion of the L7L-L11L Genes Attenuates ASFV and Induces Protection against Homologous Challenge.

ABSTRACT: African swine fever (ASF), caused by the African swine fever virus (ASFV), is a major epidemic disease endangering the swine industry. Although a number of vaccine candidates have been reported, none are commercially available yet. To explore the effect of unknown genes on the biological characteristics of ASFV and the possibility of a gene-deleted isolate as a vaccine candidate, the strain SY18ΔL7-11, with deletions of L7L-L11L genes from ASFV SY18, was constructed, and its biological properties were analyzed. The results show that deletion of genes L7L-L11L did not affect replication of the virus in vitro. Virulence of SY18△L7-11 was significantly reduced, as 11 of the 12 pigs survived for 28 days after intramuscular inoculation with a low dose (10³ TCID₅₀) or a hig

SUBMITTER: Zhang J 

PROVIDER: S-EPMC7915138 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature