AAV-Mediated CRISPRi and RNAi Based Gene Silencing in Mouse Hippocampal Neurons.

ABSTRACT: Uncovering the physiological role of individual proteins that are part of the intricate process of cellular signaling is often a complex and challenging task. A straightforward strategy of studying a protein's function is by manipulating the expression rate of its gene. In recent years, the Clustered Regularly Interspaced Short Palindromic Repeat (CRISPR)/Cas9-based technology was established as a powerful gene-editing tool for generating sequence specific changes in proliferating cells. However, obtaining homogeneous populations of transgenic post-mitotic neurons by CRISPR/Cas9 turned out to be challenging. These constraints can be partially overcome by CRISPR interference (CRISPRi), which mediates the inhibition of gene expression by competing with the transcription machinery for promote

SUBMITTER: Deutsch M 

PROVIDER: S-EPMC7915209 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature