Detection and Isolation of Emetic Bacillus cereus Toxin Cereulide by Reversed Phase Chromatography.

ABSTRACT: The emetic toxin cereulide is a 1.2 kDa dodecadepsipeptide produced by the food pathogen Bacillus cereus. As cereulide poses a serious health risk to humans, sensitive and specific detection, as well as toxin purification and quantification, methods are of utmost importance. Recently, a stable isotope dilution assay tandem mass spectrometry (SIDA-MS/MS)-based method has been described, and an method for the quantitation of cereulide in foods was established by the International Organization for Standardization (ISO). However, although this SIDA-MS/MS method is highly accurate, the sophisticated high-end MS equipment required for such measurements limits the method's suitability for microbiological and molecular research. Thus, we aimed to develop a method for cereulide toxin detection and

SUBMITTER: Kalbhenn EM 

PROVIDER: S-EPMC7915282 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature