Fabrication and Bonding of Refractive Index Matched Microfluidics for Precise Measurements of Cell Mass.

ABSTRACT: The optical properties of polymer materials used for microfluidic device fabrication can impact device performance when used for optical measurements. In particular, conventional polymer materials used for microfluidic devices have a large difference in refractive index relative to aqueous media generally used for biomedical applications. This can create artifacts when used for microscopy-based assays. Fluorination can reduce polymer refractive index, but at the cost of reduced adhesion, creating issues with device bonding. Here, we present a novel fabrication technique for bonding microfluidic devices made of NOA1348, which is a fluorinated, UV-curable polymer with a refractive index similar to that of water, to a glass substrate. This technique is compatible with soft lithography techniq

SUBMITTER: Polanco ER 

PROVIDER: S-EPMC7915968 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature