Role of Snf-β in lipid accumulation in the high lipid-producing fungus Mucor circinelloides WJ11.

ABSTRACT:

Background

Mucor circinelloides WJ11 is a high-lipid producing strain and an excellent producer of γ-linolenic acid (GLA) which is crucial for human health. We have previously identified genes that encode for AMP-activated protein kinase (AMPK) complex in M. circinelloides which is an important regulator for lipid accumulation. Comparative transcriptional analysis between the high and low lipid-producing strains of M. circinelloides showed a direct correlation in the transcriptional level of AMPK genes with lipid metabolism. Thus, the role of Snf-β, which encodes for β subunit of AMPK complex, in lipid accumulation of the WJ11 strain was evaluated in the present study.

Results

The results showed that lipid content of cell dry weight in Snf-β knockout strain was increased by

SUBMITTER: Nosheen S 

PROVIDER: S-EPMC7916304 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature