Mass Spectrometry-Based Redox and Protein Profiling of Failing Human Hearts.

ABSTRACT: Oxidative stress contributes to detrimental functional decline of the myocardium, leading to the impairment of the antioxidative defense, dysregulation of redox signaling, and protein damage. In order to precisely dissect the changes of the myocardial redox state correlated with oxidative stress and heart failure, we subjected left-ventricular tissue specimens collected from control or failing human hearts to comprehensive mass spectrometry-based redox and quantitative proteomics, as well as glutathione status analyses. As a result, we report that failing hearts have lower glutathione to glutathione disulfide ratios and increased oxidation of a number of different proteins, including constituents of the contractile machinery as well as glycolytic enzymes. Furthermore, quantitative proteomi

SUBMITTER: Tomin T 

PROVIDER: S-EPMC7916846 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature