De Novo Transcriptome Profiling of Brain Tissue from the Annual Killifish Nothobranchius guentheri.

ABSTRACT: Nothobranchius is a genus of small annual killifish found in Africa. Due to the relatively short lifespan, as well as easy breeding and care, Nothobranchius fish are becoming widely used as a vertebrate model system. Studying the genome and transcriptome of these fish is essential for advancing the field. In this study, we performed de novo transcriptome assembly of brain tissues from Nothobranchius guentheri using Trinity. Annotation of 104,271 potential genes (with transcripts longer than 500 bp) was carried out; for 24,967 genes (53,654 transcripts), in which at least one GO annotation was derived. We also analyzed the effect of a long-term food supplement with Torin 2, second-generation ATP-competitive inhibitor of mTOR, on the gene expression changes in brain tiss

SUBMITTER: Guvatova ZG 

PROVIDER: S-EPMC7916979 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature