Comprehensive Analysis of CD4⁺ T Cell Responses to CMV pp65 Antigen Restricted by Single HLA-DR, -DQ, and -DP Allotype Within an Individual.

ABSTRACT: Within an individual, six different HLA class II heterodimers are expressed co-dominantly by two alleles of HLA-DR, -DQ, and -DP loci. However, it remained unclear which HLA allotypes were used in T cell responses to a given antigen. For the measurement of the CD4⁺ T cell responses restricted by a single HLA allotype, we established a panel of artificial antigen-presenting cells (aAPCs) expressing each single HLA allele of 20 HLA-DRB1, 16 HLA-DQ, and 13 HLA-DP alleles. CD4⁺ T cell responses to cytomegalovirus (CMV) pp65 restricted by single HLA class II allotype defined in 45 healthy donors. The average magnitude of CD4⁺ T cell responses by HLA-DR allotypes was higher than HLA-DQ and HLA-DP allotypes. CD4⁺ T cell responses by DRA*01:01/DRB1*04:06

SUBMITTER: Hyun YS 

PROVIDER: S-EPMC7917246 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature