Time-Resolved, Single-Molecule, Correlated Chemical Probing of RNA.

ABSTRACT: Capturing the folding dynamics of large, functionally important RNAs has relied primarily on global measurements of structure or on per-nucleotide chemical probing. These approaches infer, but do not directly measure, through-space structural interactions. Here we introduce trimethyloxonium (TMO) as a chemical probe for RNA. TMO alkylates RNA at high levels in seconds, and thereby enables time-resolved, single-molecule, through-space probing of RNA folding using the RING-MaP correlated chemical probing framework. Time-resolved correlations in the RNase P RNA-a functional RNA with a complex structure stabilized by multiple noncanonical interactions-revealed that a long-range tertiary interaction guides native RNA folding for both secondary and tertiary structure. This unanticipated nonhiera

SUBMITTER: Ehrhardt JE 

PROVIDER: S-EPMC7917579 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature