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Transcriptome Analysis Reveals the Genes Involved in Bifidobacterium Longum FGSZY16M3 Biofilm Formation.

ABSTRACT: Biofilm formation has evolved as an adaptive strategy for bacteria to cope with harsh environmental conditions. Currently, little is known about the molecular mechanisms of biofilm formation in bifidobacteria. A time series transcriptome sequencing analysis of both biofilm and planktonic cells of Bifidobacterium longum FGSZY16M3 was performed to identify candidate genes involved in biofilm formation. Protein-protein interaction network analysis of 1296 differentially expressed genes during biofilm formation yielded 15 clusters of highly interconnected nodes, indicating that genes related to the SOS response (dnaK, groS, guaB, ruvA, recA, radA, recN, recF, pstA, and sufD) associated with the early stage of biofilm formation. Genes involved in extracellular polymeric substances were upregulated (epsH, epsK, efp, frr, pheT, rfbA, rfbJ, rfbP, rpmF, secY and yidC) in the stage of biofilm maturation. To further investigate the genes related to biofilm formation, weighted gene co-expression network analysis (WGCNA) was performed with 2032 transcript genes, leading to the identification of nine WGCNA modules and 133 genes associated with response to stress, regulation of gene expression, quorum sensing, and two-component system. These results indicate that biofilm formation in B. longum is a multifactorial process, involving stress response, structural development, and regulatory processes.

SUBMITTER: Liu Z

PROVIDER: S-EPMC7917626 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature

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Transcriptome Analysis Reveals the Genes Involved in <i>Bifidobacterium Longum</i> FGSZY16M3 Biofilm Formation.

Liu Zongmin Z Li Lingzhi L Wang Qianwen Q Sadiq Faizan Ahmed FA Lee Yuankun Y Zhao Jianxin J Zhang Hao H Chen Wei W Li Haitao H Lu Wenwei W

Microorganisms 20210214 2

Biofilm formation has evolved as an adaptive strategy for bacteria to cope with harsh environmental conditions. Currently, little is known about the molecular mechanisms of biofilm formation in bifidobacteria. A time series transcriptome sequencing analysis of both biofilm and planktonic cells of <i>Bifidobacterium longum</i> FGSZY16M3 was performed to identify candidate genes involved in biofilm formation. Protein-protein interaction network analysis of 1296 differentially expressed genes durin ...[more]

PMID: 33672820

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Project description:BackgroundStalk strength is a critical trait in maize that influences plant architecture, lodging resistance and grain yield. The developmental stage of maize, spanning from the vegetative stage to the reproductive stage, is critical for determining stalk strength. However, the dynamics of the genetic control of this trait remains unclear.ResultsHere, we report a temporal resolution study of the maize stalk transcriptome in one tropical line and one non-stiff-stalk line using 53 transcriptomes collected covering V7 (seventh leaf stage) through silking stage. The time-course transcriptomes were categorized into four phases corresponding to stalk early development, stalk early elongation, stalk late elongation, and stalk maturation. Fuzzy c-means clustering and Gene Ontology (GO) analyses elucidated the chronological sequence of events that occur at four phases of stalk development. Gene Ontology analysis suggests that active cell division occurs in the stalk during Phase I. During Phase II, processes such as cell wall extension, lignin deposition, and vascular cell development are active. In Phase III, lignin metabolic process, secondary cell wall biogenesis, xylan biosynthesis process, cell wall biogenesis, and polysaccharide biosynthetic process contribute to cell wall strengthening. Defense responses, abiotic stresses, and transport of necessary nutrients or substances are active engaged during Phase IV. Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis showed that the two maize lines presented significant gene expression differences in the phenylpropanoid biosynthesis pathway and the flavonoid biosynthesis pathway. Certain differentially expressed genes (DEGs) encoding transcription factors, especially those in the NAC and MYB families, may be involved in stalk development. In addition, six potential regulatory genes associated with stalk strength were identified through weighted gene co-expression network analysis (WGCNA).ConclusionThe data set provides a high temporal-resolution atlas of gene expression during maize stalk development. These phase-specific genes, differentially expressed genes, and potential regulatory genes reported in this study provide important resources for further studies to elucidate the genetic control of stalk development and stalk strength formation in maize.

Dataset Information

Transcriptome Analysis Reveals the Genes Involved in Bifidobacterium Longum FGSZY16M3 Biofilm Formation.

Publications

Transcriptome Analysis Reveals the Genes Involved in <i>Bifidobacterium Longum</i> FGSZY16M3 Biofilm Formation.

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