Multilocus Genotyping of Giardia duodenalis in Mostly Asymptomatic Indigenous People from the Tapirape Tribe, Brazilian Amazon.

ABSTRACT: Little information is available on the occurrence and genetic variability of the diarrhoea-causing enteric protozoan parasite Giardia duodenalis in indigenous communities in Brazil. This cross-sectional epidemiological survey describes the frequency, genotypes, and risk associations for this pathogen in Tapirapé people (Brazilian Amazon) at four sampling campaigns during 2008-2009. Microscopy was used as a screening test, and molecular (PCR and Sanger sequencing) assays targeting the small subunit ribosomal RNA, the glutamate dehydrogenase, the beta-giardin, and the triosephosphate isomerase genes as confirmatory/genotyping methods. Associations between G. duodenalis and sociodemographic and clinical variables were investigated using Chi-squared test and univariable/multivari

SUBMITTER: Koster PC 

PROVIDER: S-EPMC7917967 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature