Establishment of a Genetic Transformation System in Guanophilic Fungus Amphichorda guana.

ABSTRACT: Fungi from unique environments exhibit special physiological characters and plenty of bioactive natural products. However, the recalcitrant genetics or poor transformation efficiencies prevent scientists from systematically studying molecular biological mechanisms and exploiting their metabolites. In this study, we targeted a guanophilic fungus Amphichorda guana LC5815 and developed a genetic transformation system. We firstly established an efficient protoplast preparing method by conditional optimization of sporulation and protoplast regeneration. The regeneration rate of the protoplast is up to about 34.6% with 0.8 M sucrose as the osmotic pressure stabilizer. To develop the genetic transformation, we used the polyethylene glycol-mediated protoplast transformation, and the testing

SUBMITTER: Liang M 

PROVIDER: S-EPMC7918455 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature