BarCoder: a tool to generate unique, orthogonal genetic tags for qPCR detection.

ABSTRACT:

Background

Tracking dispersal of microbial populations in the environment requires specific detection methods that discriminate between the target strain and all potential natural and artificial interferents, including previously utilized tester strains. Recent work has shown that genomic insertion of short identification tags, called "barcodes" here, allows detection of chromosomally tagged strains by real-time PCR. Manual design of these barcodes is feasible for small sets, but expansion of the technique to larger pools of distinct and well-functioning assays would be significantly aided by software-guided design.

Results

Here we introduce barCoder, a bioinformatics tool that facilitates the process of creating sets of uniquely identifiable barcoded strains. barCoder utili

SUBMITTER: Bernhards CB 

PROVIDER: S-EPMC7919090 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature