Biochemical Validation of a Fourth Guanidine Riboswitch Class in Bacteria.

ABSTRACT: An intriguing consequence of ongoing riboswitch discovery efforts is the occasional identification of metabolic or toxicity response pathways for unusual ligands. Recently, we reported the experimental validation of three distinct bacterial riboswitch classes that regulate gene expression in response to the selective binding of a guanidinium ion. These riboswitch classes, called guanidine-I, -II, and -III, regulate numerous genes whose protein products include previously misannotated guanidine exporters and enzymes that degrade guanidine via an initial carboxylation reaction. Guanidine is now recognized as the primal substrate of many multidrug efflux pumps that are important for bacterial resistance to certain antibiotics. Guanidine carboxylase enzymes had long been annotated as urea carb

SUBMITTER: Salvail H 

PROVIDER: S-EPMC7919255 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature