A Targeted and Tuneable DNA Damage Tool Using CRISPR/Cas9.

ABSTRACT: Mammalian cells are constantly subjected to a variety of DNA damaging events that lead to the activation of DNA repair pathways. Understanding the molecular mechanisms of the DNA damage response allows the development of therapeutics which target elements of these pathways. Double-strand breaks (DSB) are particularly deleterious to cell viability and genome stability. Typically, DSB repair is studied using DNA damaging agents such as ionising irradiation or genotoxic drugs. These induce random lesions at non-predictive genome sites, where damage dosage is difficult to control. Such interventions are unsuitable for studying how different DNA damage recognition and repair pathways are invoked at specific DSB sites in relation to the local chromatin state. The RNA-guided Cas9 (CRISPR-associat

SUBMITTER: Emmanouilidis I 

PROVIDER: S-EPMC7919286 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature