Guide Cells Support Muscle Regeneration and Affect Neuro-Muscular Junction Organization.

ABSTRACT: Muscular regeneration is a complex biological process that occurs during acute injury and chronic degeneration, implicating several cell types. One of the earliest events of muscle regeneration is the inflammatory response, followed by the activation and differentiation of muscle progenitor cells. However, the process of novel neuromuscular junction formation during muscle regeneration is still largely unexplored. Here, we identify by single-cell RNA sequencing and isolate a subset of vessel-associated cells able to improve myogenic differentiation. We termed them 'guide' cells because of their remarkable ability to improve myogenesis without fusing with the newly formed fibers. In vitro, these cells showed a marked mobility and ability to contact the forming myotubes. We found that these

SUBMITTER: Ronzoni FL 

PROVIDER: S-EPMC7920023 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature