In vitro chondrogenic differentiation of human articular cartilage derived chondroprogenitors using pulsed electromagnetic field.

ABSTRACT:

Background

The ability to grow new cartilage remains the standard goal of any treatment strategy directed at cartilage repair. Chondroprogenitors have garnered interest due to their applicability in cell therapy. Pulsed electromagnetic field (PEMF) favors chondrogenesis by possible upregulation of genes belonging to TGFβ superfamily. Since TGFβ is implicated in chondrogenic signalling, the aim of the study was to evaluate the ability of PEMF to induce chondrogenesis via endogenous TGFβ production in chondroprogenitors vs differentiation using chondrogenic medium inclusive of TGFβ.

Methods

Chondroprogenitors were harvested from three non-diseased human knee joints via fibronectin assay. Passage 3 pellets were subjected to four different culture conditions: a) negative control

SUBMITTER: Vinod E 

PROVIDER: S-EPMC7920151 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature