Functional characterization and reclassification of an enzyme previously proposed to be a limonoid UDP-glucosyltransferase.

ABSTRACT:

Background

A major problem in the orange industry is 'delayed' bitterness, which is caused by limonin, a bitter compound developing from its non-bitter precursor limonoate A-ring lactone (LARL) during and after extraction of orange juice. The glucosidation of LARL by limonoid UDP-glucosyltransferase (LGT) to form non-bitter glycosyl-limonin during orange maturation has been demonstrated as a natural way to debitter by preventing the formation of limonin.

Result

Here, the debittering potential of heterogeneously expressed glucosyltransferase, maltose-binding protein (MBP) fused to cuGT from Citrus unishiu Marc (MBP-cuGT), which was previously regarded as LGT, was evaluated. A liquid chromatography - mass spectrometry (LC-MS) method was established to determine the concentrati

SUBMITTER: Cui Y 

PROVIDER: S-EPMC7920543 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature