Effects of compound probiotics and aflatoxin-degradation enzyme on alleviating aflatoxin-induced cytotoxicity in chicken embryo primary intestinal epithelium, liver and kidney cells.

ABSTRACT: Aflatoxin B₁ (AFB₁) is one of the most dangerous mycotoxins for humans and animals. This study aimed to investigate the effects of compound probiotics (CP), CP supernatant (CPS), AFB₁-degradation enzyme (ADE) on chicken embryo primary intestinal epithelium, liver and kidney cell viabilities, and to determine the functions of CP + ADE (CPADE) or CPS + ADE (CPSADE) for alleviating cytotoxicity induced by AFB₁. The results showed that AFB₁ decreased cell viabilities in dose-dependent and time-dependent manners. The optimal AFB₁ concentrations and reactive time for establishing cell damage models were 200 µg/L AFB₁ and 12 h for intestinal epithelium cells, 40 µg/L and 12 h for liver and kidney cells. Cell viabilities

SUBMITTER: Guo HW 

PROVIDER: S-EPMC7921234 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature