A choreography of centrosomal mRNAs reveals a conserved localization mechanism involving active polysome transport.

ABSTRACT: Local translation allows for a spatial control of gene expression. Here, we use high-throughput smFISH to screen centrosomal protein-coding genes, and we describe 8 human mRNAs accumulating at centrosomes. These mRNAs localize at different stages during cell cycle with a remarkable choreography, indicating a finely regulated translational program at centrosomes. Interestingly, drug treatments and reporter analyses reveal a common translation-dependent localization mechanism requiring the nascent protein. Using ASPM and NUMA1 as models, single mRNA and polysome imaging reveals active movements of endogenous polysomes towards the centrosome at the onset of mitosis, when these mRNAs start localizing. ASPM polysomes associate with microtubules and localize by either motor-driven transport or m

SUBMITTER: Safieddine A 

PROVIDER: S-EPMC7921559 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature